Revealing dynamic and elusive early-mitotic events using state-of-the-art live-cell light sheet imaging

Supervisors: Tomoyuki TanakaMichael MacDonald

Project Description:

To maintain their genetic integrity, eukaryotic cells must segregate their chromosomes properly to opposite spindle poles during mitosis. This process has important medical relevance because chromosome mis-segregation plays causative roles in human diseases such as cancers and congenital diseases. To prepare for proper chromosome segregation, kinetochores – the spindle attachment sites on chromosomes – must correctly interact with spindle microtubules (MTs) during early mitosis. It has been challenging to study these initial interactions because the dynamics of this process are complex, and the kinetochores and microtubules are densely packed and hard to distinguish. 

This research project will analyse these early kinetochore–MT interactions, in human cells, in far greater detail than has been achieved previously. To achieve this we will use a state-of-the-art TriSPIM light sheet microscope [1] that is capable of obtaining and analyzing fluorescent images of mitosis in live cells with high spatial and temporal resolution, excellent signal-to-noise and with minimal photo-damage to the dividing cells. These challenging imaging conditions are crucial to obtaining information about these vital and extremely dynamic early events in mitosis. The TriSPIM light sheet microscopy system has been recently implemented at our institute, funded by BBSRC. This is the ideal system to fulfill the above imaging conditions. 

In this project, the student will use the TriSPIM microscope to analyze kinetochore–MT interaction in human cells, under the supervision of Tanaka and MacDonald. Tanaka is an expert in chromosome segregation and kinetochore–MT interaction [2], while MacDonald is an expert in light sheet imaging [3]. Throughout this project, the student will learn basic and advanced techniques in cell and molecular biology as well as applications of state-of-the-art live-cell light sheet microscopy.

[1] Wu, Y., Chandris, P., Winter, P.W., Kim, E.Y., Jaumouillé, V., Kumar, A., Guo, M., Leung, J.M., Smith, C., Rey-Suarez, I., et al. (2016). Simultaneous multiview capture and fusion improves spatial resolution in wide-field and light-sheet microscopy. Optica 3, 897-910.
[2] Kalantzaki, M., Kitamura, E., Zhang, T., Mino, A., Novak, B., and Tanaka, T.U. (2015). Kinetochore-microtubule error correction is driven by differentially regulated interaction modes. Nat Cell Biol. 17, 421-433. 
[3] Rozbicki, E., Chuai, M., Karjalainen, A.I., Song, F., Sang, H.M., Martin, R., Knolker, H.J., MacDonald, M.P., and Weijer, C.J. (2015). Myosin-II-mediated cell shape changes and cell intercalation contribute to primitive streak formation. Nature Cell Biol 17, 397-408.

To apply for this project, please go to this link.